If you want to import everything, just leave the values as default. Next, you will need to choose Sequence Options by selecting the number of stacks to import. If you took a Z-stack image of your sample and exported the file as a series of separate images, you can import it to Fiji by selecting File > Import > Image Sequence… and opening the folder with the Z-stacks. You can cycle through channels and z-stacks using sliders below the image preview. However, the open file will be a composite image with three different channels in a Z-stack that you can cycle through. lsm file (a common format for ZEISS confocal microscopes), you can open it using the same method. For single files like this, simply go to File > Open and select your file. tif image exported from the microscope software. There are instructions for modifications included directly in the macro file. ![]() You can follow the steps below for the manual quantification, but you can also download our Fiji macro and install it by going to Plugins > Macros > Install… and then use it by selecting it from Plugins > Macros > “live dead quantification.” You might need to modify specific settings of the macro to suit your needs by going to Plugins > Macros > Edit and selecting our macro file. Green (calcein) stains for live cells, red (EthD) for dead cells. Microscope image of Live/Dead assay of neural stem cells. ![]() To follow along, you can download an example Live/Dead image here. It is available for Mac, Windows, and Linux platforms. While this approach can be used for 3D cell culture, the results are more reliable if the sample is relatively thin and there are not many cells overlapping in different focal planes.įor this analysis, we will be using free image processing software Fiji, an extended version of ImageJ. This workflow is suitable for images of 2D and thin 3D controls captured using a fluorescent or confocal microscope for samples stained with calceinAM/ethidium homodimer (EthD). This guide will walk you through a step-by-step guide on how to perform a Live/Dead quantification using Fiji. You can read more about different types of viability assays on our assays for 3D culture page. Dead cells are labeled with the ethidium homodimer dye (EthD) which binds to their DNA and fluoresces red. Live cells are stained with calcein and generate green fluorescence upon the excitation of their cytoplasm. As a consequence, darkly stained DAB has a different spectral shape than lightly stained DAB.Live/Dead assay is a very common cell staining procedure. This means that DAB does not follow the Beer-Lambert law, which describes the linear relationship between the concentration of a compound and its absorbance, or optical density. “The brown DAB reaction product is not a true absorber of light, but a scatterer of light, and has a very broad, featureless spectrum. See, e.g., the paper by CM van der Loos :
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![]() Sebastiano Venier was a Venetian general from the 16th Century who his army to victory over the Turks in the Battle of Lepanto. The names written on his weapons include Luca Traini, an Italian man who was sentenced to 12 years in prison for the drive-by shooting of six African migrants in February last year.Īlexandre Bissonnette is serving a life sentence for killing six people and injuring five others in a shooting at the Quebec Islamic Cultural Centre in 2017. Images and videos from inside a New Zealand mosque show the aftermath of a massacre that killed at least 49 people on Friday. ![]() The man is understood to have posted a manifesto online and taken to Twitter with anti-Muslim rants about birthrates and white genocide. The attacker live-streamed on his Facebook account his actions that got 49 people killed. A year on from the Christchurch mosque shootings, raw footage from the alleged gunmans video is still appearing on Facebook and Facebook-owned Instagram. The scenes appear to have been filmed with a camera mounted on his chest. There are reports of multiple casualties after a shooting at a mosque in the New Zealand city of Christchurch on Friday, here is what we know so far: Police say they are still responding to a. He flees the scene in his car in a relatively calm manner, laughing at times during the drive. He later shoots seemingly randomly on the street and returns to the car before heading back into the mosque again and repeating the process. Find out moreĬarrying a number of automatic rifles, two jerry cans and a bag with a ‘PROUDLY KIWI AS’ logo, the gunman stops his car near the mosque, takes a gun out of the boot, then walks into the building and opens fire. Sickening footage has emerged from inside a Christchurch mosque as a gunman opened fire. No equivalent chokepoint here.Inside the courtroom with Tim Clarke. Gunman’s horrifying video inside New Zealand mosque. Horrific videos like the one posted by the Christchurch mosque shooting suspect Brenton Tarrant are geared to appeal to the morbidly curious, and appeal it did. So you could grab a video and block it before the first upload attempt. Each time this happens, the companies have to spot it and create a new fingerprint."Ĭomparing the livestreamed New Zealand shooting video to content from ISIS, Stamos told ABC News, "The ISIS problem was partially cracked because the companies infiltrated all their Telegram channels. "Perceptual hashes and audio fingerprinting are both fragile, and a lot of these same kinds of people have experience beating them to upload copyrighted content. As information on the attacks started to emerge, it became increasingly clear that the shooter or shooters were adherents of far-right extremist ideology, particularly after an 87-page manifesto surfaced articulating Islamophobic and white supremacist views. But under New Zealand law, many others could face charges for spreading. YouTube and Facebook/Instagram have perceptual hashing built during the ISIS crisis to deal with this and teams looking," Stamos tweeted. Before entering a mosque in Christchurch, New Zealand, the site of one of the deadliest mass murders in the country’s history, a gunman paused to endorse a YouTube star in a. CHRISTCHURCH, New Zealand A lone white supremacist is the suspect in the Christchurch mosque killings. "So now we have tens of millions of consumers wanting something and tens of thousands of people willing to supply it, with the tech companies in between. He posted the FB Live link and mirrors to his manifesto right before, so thousands of people got copies in real-time," he tweeted. He also noted that the "shooter was an active member of a rather horrible online community (which I will not amplify) that encourages this kind of behavior. Stamos pointed out that the verboten nature of the offensive material made it a popular search on Google. liveleak new zealand mass shooting crack Facebook is one of several social media platforms scrambling to crack down on uploads of the video, which remained online for hours after the massacre. ![]() The option on the right shows a down arrow whose options are to reply, react to the message, report or delete the message.We see two available options that appear at the top of the image. ![]() Without clicking on the video, we hold the mouse icon over the video image.We will see the video in the chat ready to play but without any download option as it usually happens with photos. At first it will not be possible to download the video with any of the available options.It is important to note that the video must be fully loaded before you can download it.Open the chat containing the video you want to download and click on the video to play it.Once you have established a connection between your phone and computer, you can begin downloading videos from Whatsapp Web. Start downloading videos from Whatsapp Web Once the connection has been established, you will be able to access your Whatsapp chats on your computer.Scan the QR code using your phone’s Whatsapp app.From the whatsapp website itself it explains the steps to follow on your phone to connect it to your computer.Open the Whatsapp Web website on your computer. ![]() To download a video from Whatsapp Web you must first connect your phone to your computer through the Whatsapp Web website. Connect your phone to your computer via the Whatsapp website In this article, we will provide a step-by-step guide on how to download videos from Whatsapp Web, as well as some troubleshooting tips. However, sometimes it is necessary to download these videos from Whatsapp Web to your computer for various reasons. Whatsapp is one of the most widely used messaging apps in the world, and it is not uncommon for users to share videos with each other through the app. Ludo typically accommodates 2-4 players, each possessing four tokens. A solid grasp of Ludo’s specific rules can significantly enhance your gaming experience, whether you’re playing in person or online. You can also watch a video tutorial on how to play Ludo: Ludo online game rules with imageĮxplore the fundamental guidelines for playing Ludo, with one crucial rule being the strategic planning of moves to secure victory. Look at the Ludo game picture below to know the gameplay: However, gameplay differs between online and classic versions.Įxplore the online ludo game – Ludo Supreme. ![]() The objective remains consistent: move your tokens clockwise around the board, up the home column, and into the Home area. Ludo is a popular strategic board gamewith straightforward gameplay, now available online through mobile apps like Zupee, where you can win up to Rs.10 Lakhs. Read & find: Who invented Ludo? How to play Ludo with pictures? Ludo’s global popularity stems from its simplicity and the unpredictable twists and turns it offers, where winners can swiftly turn into losers and vice versa. Various Ludo variants were played in India for generations at family gatherings and festive occasions. Look at the Ludo picture for Chaupar board game: ![]() ![]() While the Ludo we recognize today was patented by Alfred Collier in England in 1896, its roots can be traced to a dice game called Chaupar, favored by Indian maharajas. Ludo, a timeless board game enjoyed by individuals of all ages, has a rich history that dates back centuries. I love this plugin!! i use it all the time, it's so much more intuitive to me than some regular animation programs (do i look like i know what a "shape tween" is, i just want a video of a hot dog)Ī couple small things i've noticed, the "use black as transparency" button is more like use the darkest color per frame as transparency, so if you only want a few frames to be transparent, like a door opening or a full-screen explosion, you end up with a very broken gif :\ "use exactly #000000 as transparency" fixes this and it sounds easier to implement, too, and it would open up the option for the user to choose which hex becomes transparentĪlso the frame size seemingly arbitrarily maxes out at 1000 pixels per side (and border at only 100. Previous cell dimensions and offsets are now checked to make sure they fit within the current bitmap layer.Fixed a threading issue that could cause the plugin to crash Net Animation Helper Plugin A downloadable tool for Windows Download Now Name your own price If you use Paint.This was causing a crash anytime the user tried to save individual frames or a frame strip. Guiles explains who to quickly get started in making your own positive memes with two FREE and simple to use programs: and Piv. Handle PrivilegeNotHeldException when attempting to determine Directory access rights.Remove unneeded references (that might have been causing exceptions). ![]() Installation instructions can be found in INSTALL.txt located in the zip file.Try out our Color Pick tool for Windows which is programmable and can capture any color(s) from your desktop. Just hover over the parameter in question and a pop-up will appear. It will also generate mipmaps for you, but you cant edit or view mipmaps. It will also open environment maps and 3d textures, but only the first face/depth layer will be loaded and saved. vtf files (where each frame is a different layer in Paint.NET). You can also save all frames of an animation as a single multi-frame strip, individual PNG files, or as an animated GIF(experimental). It will allow you to open and save single or multiple frame. It allows you to view any sequence of frames as an animation within an image without having to leave Paint. NET for creating sprite animations, then this plugin is for you. ![]() Once he accidentally frees the Thin Man and she drags him away from the screen once again, Six seems concerned when Mono doesn't move at first, reaching her hand out to him before he finally regains consciousness. Despite not understanding the obscure reaction Mono had to the TVs, the heroine shows genuine concern and compassion for Mono as she constantly pulls him out of the transmission he uses to get into the hallway. The girl's confidence in Mono begins to grow after he saved her from the Bullies, accepting his help when he offers to help her get back on her feet. After that, Six seems more than willing to work with Mono, remaining by his side as they travel through Wilderness and the Pale City. Although she refused at first, Six realizes that she can not traverse on her own and will require Mono's help in order to escape. It was initially thought that Mono was a special occasion for Six, as he offered his help. However, this did not save the Girl the mistress of the Nest survived, and launched herself and the Girl in the Yellow Raincoat into the ocean where they both presumably drowned. In Very Little Nightmares, it is revealed that Six is not completely indifferent to other children's need for help (or perhaps has some reciprocal moral code) as she did try to save the main character from the persecuting Pretender after the Girl herself saved Six from death. She joins forces with Mono in Little Nightmares II and, temporarily, with The Girl in the Yellow Raincoat. However, when she finds herself in a quandary, Six does seem capable of realizing what she can and cannot handle on her own. ![]() She almost never makes any attempt to help them or, as in the case of Mono, (initially) rejects any of their own help outright, demonstrating that she is concerned first and foremost with her own survival and escape. To other children littered about the world, Six is clearly distrustful. Instead, she chose to feast on the Nome, displaying a clear indifference to killing, at least while overcome by her hunger. On one occasion, a friendly Nome offered Six who was starving a sausage to eat. She has an insatiable hunger and will eat virtually anything to satisfy it, with her diet gradually becoming darker and more desperate over the course of the game. Six frequently displays the characteristics of an anti-hero she does not seem to care about saving the other children trapped aboard the Maw and will do whatever it takes to survive and escape the resort by herself, with little to no help from anyone else. Like most characters in the Little Nightmares series, Six does not speak however, her personality can be inferred through her actions. Little is known about Six's past before the games and comics' events, but it's stated that she "belongs elsewhere", implying that she used to live somewhere else before the events in the games and comics. Her face is hidden by her grown-out hair, and stretched-out raincoat. Her body is completely warped and lanky, with her left foot seemingly dislocated judging by her structure. When Mono finally finds Six in the Black Tower, he discovers her to have been changed into a distorted, monstrous creature with long arms resembling the Janitor's. In Little Nightmares II, Six wears a charcoal shirt under a button-up, stained, gray cardigan that reaches to her knees instead of her trademark raincoat, which she later wears over the cardigan after she escapes the School with Mono. This appearance of Six is similar to that of her in the concept art of the six children. Her hair also looks neatly combed compared to other designs of her in other Little Nightmares media. In Very Little Nightmares she wore a white, short-sleeved shirt and matching shorts. Up close, she has messy black bob-cut hair with long bangs that completely obscure the top half of her face, only revealing her pointed chin and tiny nose and mouth. She wears a bright yellow raincoat that ends just above her knees with three buttons, two pockets, and a rhombus-shaped hood. Six is much smaller than the adult characters of the series, being barely a third of their height with an extremely small, thin frame. She has already seen more than any normal child ever should, but then. Little Nightmares II DescriptionĪwaking in a world she cannot recognise, Six must learn to trust someone else if she is to stand a chance of survival. She’s smart, and tough, and has a lovely yellow raincoat. Most children would have already given up, but Six is different. Trapped in the darkest depths of The Maw, starving and alone, Six’s world is one filled with danger. Website Description Little Nightmares Description 10 Little Nightmares (2017 comic series). ![]() For example, if you are using the beam to span above bi-fold doors you may need to set a lower limit, or if the beam is going to be used as a structural ridge beam the variable load deflection should not normally exceed 10mm.Ħ.2) Total Variable and Permanent Load Deflection Limit Normally this is restricted to span/360, however there are exceptions. Limits, but you may need to change these. Our Calculator defaults to the recommended ![]() Limit for how much it can sag under Variable Loads – usually things that canĬhange, such as people or furniture – and under Permanent Loads, such as the The distribution of bending moments along the length of the beam affects it buckling capacity, C1 is a factor for the shape of the bending moment between points of lateral restraint, for a beam with only uniformly distributed loads along its full length C1 can be taken as 1.13, for more information see SCI Publication P360.Ī Deflection Limit is the maximum amount you allow the beam to sag. This is the most conservative, safe value and is recommended for general use. Recommended value from EN 1990:2002+A1 Eurocode – Basis of structural design (UK National annex) is 1.35 and our Calculator defaults to this. In the calculations all the permanent loads are multiplied by this safety factor to ensure a safe design. Permanent loads (also known as dead loads) are usually things that do not change, such as the weight of the floor or a wall. Recommended value from EN 1990:2002+A1 Eurocode – Basis of structural design (UK National annex) is 1.5 and our Calculator defaults to this. In the calculations all the imposed loads are multiplied by this safety factor to ensure a safe design. Imposed loads (also known as live loads) are usually things that can change, such as people or furniture. You can also enter a buckling length, for example where the ends of the beams are not fully restrained you can input a buckling length that is greater than the effective span, for more information on buckling lengths please refer to SCI Publication P360. If there is more than one restraint, you will need to add these as well. In this case, you should measure the dimension between the lateral restraint and the beam end and enter this dimension. If the beam is restrained at points along its length, for example where another beam is fixed at right angles to the beam along its length. If your beam is not going to be fully restrained and the beam is only restrained at its supports, you can select, ‘Unrestrained – the beam is only restrained at its support’. If you need to know more, please call 01332 410066 for help. It is only classedĪs fully restrained if it meets the requirements stated in SCI Publication P360, an exampleīeing where a steel beam is cast into a concrete floor. If you need to work out the point load from a beam which is going to be supported by another beam, please see our How to design a beam which is required to support another beam guide.Ĥ.1) You need to know if the beam is going to be Fully Restrained along its length. If the load on your beam is a Point Load, you will need to enter the dimension between the Point Load and the end of the beam, as well as its Permanent and Variable load. You do not need to add the weight of the steel beam itself, our calculator automatically does this. We also have a list of Standard permanent loadsįor commonly used items, such as clay tiles and rafters. ![]() "Sloping roof with an angle of up to 30 degrees or timber floor in a domestic dwelling" and then enter the Width or Height of the load (use "other" if you can't see an option that describes the nature of your load.)įor details on how to calculate the load widths, see our Examples Page and Diagrams showing how to calculate load widths. If you choose one of these types, you can select the Loading Details from the Dropdown Menu (e.g. Point Load is where there are localised loads at certain points along the beam.ģ.1) Uniformly Distributed Loads and Partial Uniformly Distributed Loads.Partial Uniformly Distributed Load is where the load is evenly distributed across part of the beam.Uniformly Distributed Load is where the load is evenly distributed across the full length of the beam.You need to know what type of load your beam will be supporting. ![]() You can learn more about how we ensure our content is accurate and current by reading our editorial policy. We link primary sources - including studies, scientific references, and statistics - within each article and also list them in the resources section at the bottom of our articles. Medical News Today has strict sourcing guidelines and draws only from peer-reviewed studies, academic research institutions, and medical journals and associations. This may support the concept of DVZs and the notion that these sensitive areas may contribute to orgasm in some women. However, it does not necessarily mean that the A-spot exists in all or most women. This suggests that the stimulation of the front wall of the vagina may be pleasurable. Although 66.7% of 45 women either reached orgasm or requested to stop before reaching orgasm during the stimulation of the front wall of the vagina, the researchers did not identify specific spots that offered greater benefits. ![]() However, the study did not find evidence for specific erogenous spots.Ī 1984 study sought to investigate erogenous zones on the front wall of the vagina. In the study, 89% of 27 participants reached orgasm due to the stimulation of these areas. Some studies suggest that most women have erogenous zones near the purported location of the A-spot.įor example, one 1985 study found that the upper front wall and bottom back wall of the vagina were the most common vaginal erogenous zones. Some researchers argue that there is not sufficient scientific evidence to confirm that it exists, especially not in all women. However, researchers have not extensively studied this zone in large samples of women. Thanks to Dr Charlotte Proby for her permission and the photography.In theory, anyone born with a vagina may have an AFE zone. They usually occur on areas of skin exposed to the sun like the scalp or ear. Squamous cell skin cancers can vary in how they look. It might look skin coloured, waxy, like a scar or thickened area of skin that's very slowly getting bigger. Pronounced mor-fee-ic, this type of basal cell skin cancer may look like a sore area on the skin that doesn’t heal. They can look like warts or sometimes a melanoma. Pigmented basal cell cancers have dark areas, often brown, blue or grey in colour. Sometimes they have a sore (ulcerated) area and it may also have fluid filled sacs (cystic). ![]() You can often also see their blood vessels. Nodular basal cell cancers can look see through (translucent) and shiny. morphoeic basal cell skin cancer - also known as sclerosing or infiltrating basal cell skin cancer.There are different types of basal cell skin cancers. You can then show these pictures to your doctor. This gives you a more accurate idea about its size and can help you tell if it's changing. If you can it's a good idea to put a ruler or tape measure next to the abnormal area when you take the photo. You can take a photo of anything that doesn't look quite right. This is very important if you're regularly outside in the sun for work or leisure. Or you could get your partner or a friend to look. To look at areas you can’t see easily, you could try using a hand held mirror and reflect your skin onto another mirror. That way, you'll notice any changes more easily. To spot skin cancer early it helps to know how your skin normally looks. ![]() Non melanoma skin cancer tends to develop most often on skin exposed to the sun. So it's important you visit your GP as soon as possible if you notice a change in your skin. The earlier a skin cancer is diagnosed, the easier it is to treat. Russian Freecell - somewhat similar to regular freecell except only around half the cards are turned over initially & a freecell is removed after each round.Penguin - like a suit-based modified freecell with more free cells & the need to build foundations from an arbitrary number in the middle of the deck.Medieval Freecell - see how quickly you can beat a random level or choose to play any 1,000,000 different levels.Freecell Summer Holiday - tropical themed single-round game with a 10-minute limit.Freecell Klondike - This is a hybrid between the two games with a single level and a 7-minute time limit.As you advance through the levels each level has one fewer free cell than the level prior. Freecell Extreme - 4-level game with a 15-minute time level on each.Cards are dealt to 12 piles on the tableau. This game has a single 20-minute level with 8 foundations and 8 free cells. Freecell Duplex - think of this game as double Freecell.Freecell Christmas - single deck 10-minute game with beautiful holiday aesthetic and music.Free Cell - no frills basic single-level game with a 30 minute time limit.Classic Freecell - single-level 10 minute game.Blind Freecell - all cards on the tableau above the bottom row are dealt face down & 4 cards are dealt to the free cells.We also have a version of Freecell Solitaire which allows you to submit your score and see how you performed compared to other players. game has 4 free cells you can temporarily store cards in. 35 cards are dealt to 7 columns in the tableau and the remaining 17 cards are across the top and can be used at any point in time. Flower Garden - single-level single-deck game where you can only move a single card at a time, but the tableau is not suit dependent.Flower - timed 3-level game which is somewhat conceptually similar to La Belle Lucie but far easier as you can move stacks of descending cards so long as they alternate suits. ![]() ![]() Egypt - 100 level game where you make pairs using cards on the tableau or from within the reserve.Double - compete against a computer player in a timed game of Klondike where you both can lay cards to each other's foundations.We offer another version of Crescent Solitaire with 4 timed levels with increasing difficulty on each. Crescent - large tableau where you can stack cards by suit in ascending or descending order until moving them across to the 8 foundations.Classic - timed 3-level game which is turn 1, turn 2 then turn 3 as you progress through the rounds. ![]()
![]() 5) Big Sur 2 macOS Theme for Windows 10Ĭomes from the developer of the macOS X theme (third in this list), the theme itself vouches for the quality. Don’t want to go that far? Don’t worry! You’re free to choose other themes in this list. For that, you will have to bag the paid version. The free or Lite version doesn’t change the icons. There are two versions available paid and free. First, uninstall it and then check this out. 4) macOS Monterey SkinPackĭon’t jump into installing this macOS skin for windows 10 right away if you already have another one on your system. On the download page, you can see appreciation comments from hundreds of users, which are proof that the skin pack works just well. And, you will have to create an account on Devian to download the theme.įor an optimum experience, the developer recommends the users grab four third-party applications UltraUXThemePatcher, OldNewExplorer, OneSettings, and StartIsBack. The one you are getting here turns your computer into the version, Snow Leopard. Just like Microsoft does for Windows, Apple also releases multiple iterations of macOS. In case you think you will botch up everything, create a restore point first. The latest version has brought three changes High Sierra and Mojave wallpapers, updated Resource Hacker version, and changed default framework to Aero theme. That’s the aim, right? By changing the sounds, cursors, fonts, and even the Finder bar, the theme emulates the perfect macOS environment. Here comes yet another complete Mac look like skin for Windows. So, what are you waiting for? Given that you can always choose to opt-out, don’t hesitate to get your hands on it. On the download page (where you can also find the zip file password), they say they release regular updates to fix bugs. The developers have tried their best to mimic everything that’s there on the original OS from Apple (be it the login screen, icons, file explorer, or the dock).Įven the boot screen will imitate Apple once you install Sierra SkinPack. When you install this theme pack, you will blame yourself for not getting it earlier. Is your patience wearing thin? Here the list goes. We have chosen ten of the best MacBook themes from many available on the web. Once you remove the program, everything will revert to being normal.Īlso Read: How to Run Android Applications on MAC 10 Best macOS Skins for Windows 10 Phew!Ĭaution :- If you are running a PC with limited resources (say, for example, a computer with low memory), the performance may decrease after installing the theme. Open the Control Panel and go to Programs and Features. You should do that to see the appearance changes in action.īingo! You now know installing a Mac theme on your Windows computer is as easy as installing any other application. Step 4: After installing all icons, patcher etc (depending on the theme files) It will ask you to restart your system if not then also restart your system. Follow on-screen instructions and it won’t take more than five minutes to finish the installation. Step 3: You will find various files like themes, wallpapers. So, you will have to unzip the contents into a folder of your convenience after downloading (in search of a file extractor, our choice is WinRar). Don’t get confused! Use our next section to select a theme. Step 1: It’s obvious, isn’t it? You have to download the installer. How to Install a Mac Theme on Your Windows 10 Computer? So first, we are going to have a look at the steps to install the theme. Knowing to use the skin pack is as important as downloading it. You can’t turn your Windows PC into a Mac machine just by downloading a theme. ![]() MacOS X, El Capitan, Sierra, Big Sur, Monterey, Catalina with these themes pack. Transform your Windows 10 and Windows 11 into MacOS various versions such as. However, handpicking the best from a lot is going to be a headache. A simple Google search will get you many such themes at your fingertips. |
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